Review of Research Paper on Cold Perecption via TRPM8 -- Biology Nerve

Introduction: The first reaction to a minor wound or sprain is to apply ice. Cold's analgesic effects are a profound and under appreciated phenomenon. The nerve providing the sensation we know as cold acts to inhibit the "pain pathway." This wonderful nervous pathway is called TRPM8. This study indicates that the ion channel called "transient receptor potential melastatin 8", or TRPM8, is a primary component of cold sensation. Cultured nerve fibers devoid of this ion channel have considerably less response to the sub-ambient temperatures and cold-simulating chemicals that these nerves normally respond to fervently. Mice that have been genetically deprived of the TRPM8 channel also display a noticeable decrease in non-noxious cold sensitivity. Essentially without this component of the nerve, mice don’t notice mild cold, and actually have no particular preference to warmth. This appears to be true only for non-noxious temperatures (roughly above 12C in this case). Below that threshhold, it is thought that cold is perceived by thermal nociceptors as pain due to the extreme and potentially harmful aspects of severe cold. Methods: The analysis of this ion channel was observed on both the cellular and behavioral perspectives in an attempt to identify the biochemical properties and characteristics of the channel and the ramifications thereof in a model organism. Methods: Gene Deletion Overview: Gene Deletion: The researchers deleted a significant portion of the TRPM8 gene. This was done through PCR, digestion, and ligation, effectively stopping the production of TRPM8 ion channel. PCR or polymerase chain reaction is a process which uses enzymes and primers to amplify or modify a DNA sequence. Deletion is accomplished by ... ...ficient, producing concurrent and decisive data. TRPM8 plays a vital role in sesation of its temperature range. These observations lead to the fact that all senastion is a balance of multiple pathways. Any perceived stimulus is actually a complex combination of neurons all being stimulated differently and decifered by the brain. So when one is exposed to cold, the sensory cortex is actully receiving stimulus of the absence of heat, the pain, the local vascular changes, and cold each delivered by a specific neuron. This must be true for all sensations. Further definition of this fact could lead to break throughs in pain management, virtual reality, artificial flavor and scent stimulation, physical therapy or perhaps futuristic massage devices. Sources Nature magazine article "The menthol receptor TRPM8 is the principal detector of environmental cold "